Effect of Different Concentration of Ri Plasmid on Division of Electrotreated Cell Suspension Culture derived from Stem Callus of Vicia faba L. Plants Embedded in Agar Multiple Drops

Samples of cell suspension represents seven different densities were exposed to three electrotreatment 40, 50, 60 volt and for a period time 100 msec then the mixed with concentration Ri plasmid 25, 75 Microliter. The samples cultured by embedding in agar drops solid-liquid cultures. Results proved that enhanced division caused clear increase microcolonies, total number colonies reached 3276 when culture was 50v / msec., these developed microcalli percentage 90%. Callus from light green color friable texture.